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picolorlock gold phosphate detection system  (SYGNIS AG)

 
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    Structured Review

    SYGNIS AG picolorlock gold phosphate detection system
    Picolorlock Gold Phosphate Detection System, supplied by SYGNIS AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/picolorlock+gold+phosphate+detection+system/picolorlock+gold+phosphate+detection+system/pmc11648822-462-7-12
    Average 90 stars, based on 1 article reviews
    picolorlock gold phosphate detection system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Protein Concentration:

    Article Title: Mesenchymal Stem Cell Exosomes Enhance Posterolateral Spinal Fusion in a Rat Model
    Article Snippet: The conditioned medium was size-fractionated using tangential flow filtration and concentrated 50 × using a membrane with a 100-kDa molecular weight cut-off (Sartorius, Göttingen, Germany). .. MSC exosome preparation was assayed for protein concentration using a Coomassie Plus protein assay (Thermofisher Scientific, USA), particle size concentration and distribution by ZetaView (Particle Metrix GmbH, Munich, Germany), and CD73/ecto-5′-nucleotidase (NT5E) activity using a PiColorLock Gold Phosphate Detection System (Innova Biosciences, Cambridge, UK) in accordance with the Minimal Information for Studies of Extracellular Vesicles (MISEV) [ , ], and specifically with the identity and potency metrics proposed for MSC-EV preparations [ , ]. ..

    Concentration Assay:

    Article Title: Mesenchymal Stem Cell Exosomes Enhance Posterolateral Spinal Fusion in a Rat Model
    Article Snippet: The conditioned medium was size-fractionated using tangential flow filtration and concentrated 50 × using a membrane with a 100-kDa molecular weight cut-off (Sartorius, Göttingen, Germany). .. MSC exosome preparation was assayed for protein concentration using a Coomassie Plus protein assay (Thermofisher Scientific, USA), particle size concentration and distribution by ZetaView (Particle Metrix GmbH, Munich, Germany), and CD73/ecto-5′-nucleotidase (NT5E) activity using a PiColorLock Gold Phosphate Detection System (Innova Biosciences, Cambridge, UK) in accordance with the Minimal Information for Studies of Extracellular Vesicles (MISEV) [ , ], and specifically with the identity and potency metrics proposed for MSC-EV preparations [ , ]. ..

    Activity Assay:

    Article Title: Mesenchymal Stem Cell Exosomes Enhance Posterolateral Spinal Fusion in a Rat Model
    Article Snippet: The conditioned medium was size-fractionated using tangential flow filtration and concentrated 50 × using a membrane with a 100-kDa molecular weight cut-off (Sartorius, Göttingen, Germany). .. MSC exosome preparation was assayed for protein concentration using a Coomassie Plus protein assay (Thermofisher Scientific, USA), particle size concentration and distribution by ZetaView (Particle Metrix GmbH, Munich, Germany), and CD73/ecto-5′-nucleotidase (NT5E) activity using a PiColorLock Gold Phosphate Detection System (Innova Biosciences, Cambridge, UK) in accordance with the Minimal Information for Studies of Extracellular Vesicles (MISEV) [ , ], and specifically with the identity and potency metrics proposed for MSC-EV preparations [ , ]. ..

    Article Title: A 4-cyano-3-methylisoquinoline inhibitor of Plasmodium falciparum growth targets the sodium efflux pump PfATP4
    Article Snippet: .. ATPase activity was determined by measuring the production of P i from ATP hydrolysis, using the PiColorLock Gold Phosphate Detection System (Innova Biosciences). .. The duration of the reactions was 10 min and the reaction mixtures had a pH of 7.2 and contained 20 mM KCl, 2 mM MgCl 2 , 50 mM Tris, 150 mM NaCl or choline chloride, parasite membrane (final concentration of total protein was 50 μg/mL), the compound(s) of interest (final [DMSO] in each reaction = 0.5% v/v) and 1 mM ATP (Na 2 ATP,3H 2 O; MP Biomedicals; added last to initiate the reaction).

    Article Title: A pH Fingerprint Assay to Identify Inhibitors of Multiple Validated and Potential Antimalarial Drug Targets.
    Article Snippet: .. Membranes were prepared from saponin-isolated trophozoite-stage parasites (3D7 strain), their protein content determined using a Bradford assay, and ATPase activity measured using a PiColorLock Gold Phosphate Detection System (Innova Biosciences), as described previously.60 The final reactions contained 150 mM choline chloride, 50 mM Tris, 20 mM KCl, 2 mM MgCl2, 50 μg/mL (total) membrane protein, the compound of interest or solvent alone (introducing 0.4% v/v DMSO), and 1 mM ATP (Na2ATP.3H2O; MP Biomedicals) and had a pH of 7.2. ..

    Membrane:

    Article Title: Property and Activity Refinement of Dihydroquinazolinone-3-carboxamides as Orally Efficacious Antimalarials that Target PfATP4
    Article Snippet: .. Protein concentrations in the membrane preparations were determined using a Bradford assay, and the ATPase assays were conducted using a PiColorLock Gold Phosphate Detection System (Innova Biosciences), as described previously. ..

    Article Title: A pH Fingerprint Assay to Identify Inhibitors of Multiple Validated and Potential Antimalarial Drug Targets.
    Article Snippet: .. Membranes were prepared from saponin-isolated trophozoite-stage parasites (3D7 strain), their protein content determined using a Bradford assay, and ATPase activity measured using a PiColorLock Gold Phosphate Detection System (Innova Biosciences), as described previously.60 The final reactions contained 150 mM choline chloride, 50 mM Tris, 20 mM KCl, 2 mM MgCl2, 50 μg/mL (total) membrane protein, the compound of interest or solvent alone (introducing 0.4% v/v DMSO), and 1 mM ATP (Na2ATP.3H2O; MP Biomedicals) and had a pH of 7.2. ..

    Article Title: Lactam Truncation Yields a Dihydroquinazolinone Scaffold with Potent Antimalarial Activity that Targets PfATP4
    Article Snippet: Values were normalized to DMSO control as a percentage and EC 50 and dose response curves generated using GraphPad Prism. .. Membrane ATPase assays were performed using a PiColorLock Gold Phosphate Detection System (Innova Biosciences), essentially as described previously. ..

    Bradford Assay:

    Article Title: Property and Activity Refinement of Dihydroquinazolinone-3-carboxamides as Orally Efficacious Antimalarials that Target PfATP4
    Article Snippet: .. Protein concentrations in the membrane preparations were determined using a Bradford assay, and the ATPase assays were conducted using a PiColorLock Gold Phosphate Detection System (Innova Biosciences), as described previously. ..

    Article Title: A pH Fingerprint Assay to Identify Inhibitors of Multiple Validated and Potential Antimalarial Drug Targets.
    Article Snippet: .. Membranes were prepared from saponin-isolated trophozoite-stage parasites (3D7 strain), their protein content determined using a Bradford assay, and ATPase activity measured using a PiColorLock Gold Phosphate Detection System (Innova Biosciences), as described previously.60 The final reactions contained 150 mM choline chloride, 50 mM Tris, 20 mM KCl, 2 mM MgCl2, 50 μg/mL (total) membrane protein, the compound of interest or solvent alone (introducing 0.4% v/v DMSO), and 1 mM ATP (Na2ATP.3H2O; MP Biomedicals) and had a pH of 7.2. ..

    Solvent:

    Article Title: A pH Fingerprint Assay to Identify Inhibitors of Multiple Validated and Potential Antimalarial Drug Targets.
    Article Snippet: .. Membranes were prepared from saponin-isolated trophozoite-stage parasites (3D7 strain), their protein content determined using a Bradford assay, and ATPase activity measured using a PiColorLock Gold Phosphate Detection System (Innova Biosciences), as described previously.60 The final reactions contained 150 mM choline chloride, 50 mM Tris, 20 mM KCl, 2 mM MgCl2, 50 μg/mL (total) membrane protein, the compound of interest or solvent alone (introducing 0.4% v/v DMSO), and 1 mM ATP (Na2ATP.3H2O; MP Biomedicals) and had a pH of 7.2. ..



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